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Related Experiment Video

Updated: Oct 23, 2025

$Microfluidic Chips for In Situ Crystal X-ray Diffraction and In Situ Dynamic Light Scattering for Serial Crystallography$

Microfluidic Chips for In Situ Crystal X-ray Diffraction and In Situ Dynamic Light Scattering for Serial Crystallography

Published on: April 24, 2018

A capillary-based microfluidic device enables primary high-throughput room-temperature crystallographic screening.

Shuo Sui¹, Anne Mulichak², Raviraj Kulathila³

¹Department of Chemical Engineering, University of Massachusetts Amherst, Amherst, MA, USA.

Journal of Applied Crystallography

|August 25, 2021

Summary

A new microfluidic chip speeds up small-molecule screening using room-temperature X-ray crystallography. This method allows for efficient identification of novel compound binding events by analyzing protein crystals.

Keywords:

X-ray diffraction compound screening microfluidics protein crystallography structural biology

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Author Spotlight: High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography

Author Spotlight: High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography

Published on: March 10, 2023

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Last Updated: Oct 23, 2025

$Microfluidic Chips for In Situ Crystal X-ray Diffraction and In Situ Dynamic Light Scattering for Serial Crystallography$

Microfluidic Chips for In Situ Crystal X-ray Diffraction and In Situ Dynamic Light Scattering for Serial Crystallography

Published on: April 24, 2018

Crystallization and Structural Determination of an Enzyme:Substrate Complex by Serial Crystallography in a Versatile Microfluidic Chip

Crystallization and Structural Determination of an Enzyme:Substrate Complex by Serial Crystallography in a Versatile Microfluidic Chip

Published on: March 20, 2021

Author Spotlight: High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography

Author Spotlight: High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography

Published on: March 10, 2023

Area of Science:

Structural Biology
Biophysics
Materials Science

Background:

X-ray crystallography is crucial for determining protein structures.
Current screening methods can be time-consuming and require specific conditions.
Accelerating compound screening can lead to faster drug discovery.

Purpose of the Study:

To develop a novel microfluidic strategy for rapid small-molecule compound screening.
To enable room-temperature X-ray crystallography screening of protein crystals.
To facilitate high-throughput screening for novel binding events.

Main Methods:

Fabrication of ultra-thin microfluidic devices using UV-curable polymer and photolithography.
Integration of capillary channels within the microfluidic chips.
Utilizing 3D-printed frames for mechanical stability and robotic compatibility.
Implementing an in situ crystal-soaking screening workflow.

Main Results:

The microfluidic chips demonstrated ease of sample manipulation and stability.
Minimal X-ray background was achieved with the designed devices.
Quantitative electron density maps were efficiently obtained, enabling detection of weak binding events.
The workflow is compatible with automated liquid and sample handling.

Conclusions:

The developed microfluidic strategy significantly accelerates small-molecule compound screening.
This approach enables efficient discovery of novel binding events at high concentrations.
The method paves the way for automated, high-throughput protein crystallography screening at synchrotron sources.