Statistical optimization of thermostable alkaline protease from Bacillus cereus KM 05 using response surface...

Area of Science:

• Enzymology
• Industrial Microbiology

Background:

• Proteases are crucial industrial enzymes with widespread applications.
• Their production and optimization are vital for various sectors, including detergents.

Purpose of the Study:

• To isolate a bacterium with high alkaline protease activity.
• To optimize protease production using Response Surface Methodology (RSM).

Main Methods:

• Isolation and identification of protease-producing bacteria from detergent-contaminated soil.
• Optimization of production conditions using statistical methods, including Box-Behnken design.
• Characterization of protease stability and compatibility with commercial detergents.

Main Results:

• Bacillus cereus KM05 was identified, producing alkaline protease with 22 U/ml activity.
• Optimization increased protease activity by 1.5-fold to 28.6 U/ml.
• The protease demonstrated thermotolerance, alkaline pH stability, and 97% stain removal efficiency.

Conclusions:

• The isolated alkaline protease is stable and effective for stain removal.
• This protease is a promising candidate for detergent formulations.