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Updated: Aug 11, 2026

Analysis of Cell Migration within a Three-dimensional Collagen Matrix
08:02

Analysis of Cell Migration within a Three-dimensional Collagen Matrix

Published on: October 5, 2014

Methodology for detection of heterogeneity of cell locomotory phenotypes in three-dimensional gels

E D Shields1, P B Noble

  • 1Department of Oral Biology, Faculty of Dentistry, McGill University, Montreal, Canada.

Experimental Cell Biology
|January 1, 1987
PubMed

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Two-dimensional Gel Electrophoresis01:22

Two-dimensional Gel Electrophoresis

Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as  cells...