Gas chromatographic analysis of monoalkylhydrazines

A quantitative electron-capture gas chromatographic assay procedure was developed for the analysis of monoalkylhydrazines in biological samples. Application to the analysis of phenelzine was demonstrated. Four monoalkylhydrazines were analyzed in whole blood by reaction with pentafluorobenzaldehyde to form stable hydrazone derivatives which were extracted and subsequently reacted with pentafluoropropionic anhydride to give products which were very sensitive to electron-capture detection when analyzed by gas chromatography. Methylhydrazine, benzylhydrazine, phenelzine and pheniprazine each yielded single derivatives with this procedure suggesting that the analytical procedure has a broad application to the analysis of other monoalkylated hydrazines. The method was applied to monitor whole blood levels of phenelzine in rats treated intravenously with phenelzine sulphate.