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Determination of acetylcholine and choline in microdialysates from spinal cord of rat using liquid chromatography
1Department of Analytical Chemistry, Uppsala University, Sweden.
Journal of Chromatography
|December 25, 1987
Abstract:
A method for the determination of acetylcholine and choline in microdialysates from rat spinal cord has been developed using liquid chromatography, enzyme-catalysed post-column derivatization and electrochemical detection. The sensitivity required was achieved by a certain degree of miniaturization, careful temperature control and a slight modification of the amperometric detector. Determinations were performed on 5-microliter samples.