A Warp-Knitted Light-Emitting Fabric-Based Device for In Vitro Photodynamic Therapy: Description, Characterization,
Elise Thécua1,2, Laurine Ziane1, Guillaume Paul Grolez1
1U1189-ONCO-THAI-Laser Assisted Therapy and Immunotherapies for Oncology, CHU Lille, Inserm, Univ. Lille, F-59000 Lille, France.
Cancers
|August 27, 2021
Summary
A novel CELL-LEF device using knitted light-emitting fabrics enables effective in vitro photodynamic therapy (PDT). This system delivers low irradiance over extended periods, significantly reducing tumor cell viability in cancer cell lines.
Area of Science:
- Biomedical Engineering
- Photomedicine
- Materials Science
Background:
- Photodynamic therapy (PDT) shows promise but faces challenges due to complex parameters.
- A need exists for user-friendly devices for in vitro PDT applications.
Purpose of the Study:
- To present and characterize a novel optical device, CELL-LEF, for in vitro PDT.
- To demonstrate the efficacy of 5-ALA-mediated PDT using CELL-LEF on human cancer cell lines.
Main Methods:
- Development and technical characterization of CELL-LEF, a device using knitted light-emitting fabrics.
- In vitro cytotoxic study using 5-aminolevulinic acid (5-ALA) mediated PDT on HepG2 and U87 cancer cell lines.
- Evaluation of irradiance delivery (1 mW/cm²) over a large area (750 cm²) and temperature stability (mean 35.1 °C).
Main Results:
- CELL-LEF achieved the target irradiance of 0.99 mW/cm² with minimal deviation.
- The device maintained stable temperature, crucial for in vitro experiments.
- 5-ALA PDT with CELL-LEF significantly reduced tumor cell viability, with nearly complete cell death in HepG2 cells.
Conclusions:
- CELL-LEF is a suitable device for in vitro PDT applications requiring low irradiance and long illumination periods.
- The study validates the potential of CELL-LEF for standardized and effective PDT research.
- This technology facilitates further investigation into PDT mechanisms and therapeutic strategies.
