Oxidative Power: Tools for Assessing LPMO Activity on Cellulose.
Federica Calderaro1,2, Loes E Bevers1, Marco A van den Berg1
1DSM Biotechnology Center, 2613 AX Delft, The Netherlands.
Biomolecules
|August 27, 2021
Summary
Lytic polysaccharide monooxygenases (LPMOs) boost lignocellulosic hydrolysis, but measuring their activity is challenging. This review details current methods for assessing LPMO activity, highlighting their potential and limitations for industrial applications.
Area of Science:
- Biochemistry
- Enzymology
- Biotechnology
Background:
- Lytic polysaccharide monooxygenases (LPMOs) are crucial enzymes in lignocellulose degradation.
- Their boosting effect enhances the efficiency of cellulolytic enzymes in biomass hydrolysis.
- Industrial applications necessitate reliable methods for LPMO activity assessment.
Purpose of the Study:
- To provide a comprehensive overview of current methods for measuring LPMO activity.
- To discuss the potential and limitations of each method.
- To offer practical examples for experimental design and data interpretation.
Main Methods:
- Review of existing literature on LPMO activity assays.
- Analysis of factors influencing LPMO activity measurements (co-substrates, electron donors, inhibitors, substrate heterogeneity).
- Discussion of assay methodologies and their applicability.
Main Results:
- Several methods exist for LPMO activity assessment, each with specific advantages and drawbacks.
- Accurate measurement requires careful consideration of experimental conditions and potential interfering factors.
- Practical examples illustrate the application and interpretation of assay results.
Conclusions:
- Standardized and reliable methods for LPMO activity measurement are essential for industrial biotechnology.
- Understanding assay limitations is key to successful application of LPMOs in biomass conversion.
- Further research may lead to improved and more accessible LPMO activity assays.
