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Related Experiment Video

Updated: Oct 22, 2025

A Suppressor Screen for the Characterization of Genetic Links Regulating Chronological Lifespan in Saccharomyces cerevisiae

A Suppressor Screen for the Characterization of Genetic Links Regulating Chronological Lifespan in Saccharomyces cerevisiae

Published on: September 17, 2020

Longevity Regulation by Proline Oxidation in Yeast.

Akira Nishimura¹, Yuki Yoshikawa¹, Kazuki Ichikawa¹

¹Division of Biological Science, Graduate School of Science and Technology, Nara Institute of Science and Technology, Nara 630-0192, Japan.

|August 27, 2021

Summary

Proline oxidation by Put1 is crucial for yeast longevity. This process maintains mitochondrial function and energy production during aging, extending cellular lifespan.

Keywords:

Saccharomyces cerevisiae chronological lifespan energy metabolism homeostasis longevity proline proline oxidase

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Area of Science:

Biochemistry
Cellular Biology
Gerontology

Background:

Proline is a versatile amino acid vital for cellular homeostasis, serving as a nitrogen and energy source and stress protectant.
Proline metabolism plays a key role in maintaining cellular balance.

Purpose of the Study:

To investigate the role of proline oxidation in yeast chronological lifespan.
To elucidate the mechanism by which proline metabolism influences cellular aging.

Main Methods:

Yeast strains with deletions in PUT1 and PUT3 genes were utilized.
Chronological lifespan assays were performed with and without proline supplementation.
Mitochondrial membrane potential and ATP production were measured during aging.

Main Results:

Deletion of PUT1, encoding proline oxidase, significantly reduced yeast chronological lifespan.
Proline supplementation extended the lifespan of wild-type yeast but not PUT1-deleted strains.
PUT1 induction by the transcriptional factor Put3 occurs during yeast aging, and PUT3 deletion shortened lifespan.
Proline oxidation by Put1 maintained mitochondrial membrane potential and ATP production throughout aging.

Conclusions:

Mitochondrial energy metabolism is sustained by the oxidative degradation of proline.
This proline oxidation pathway is a critical regulator of yeast longevity.