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Investigating Interactions Between Histone Modifying Enzymes and Transcription Factors in vivo by Fluorescence Resonance Energy Transfer
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Heterodimer formed by ROC8 and ROC5 modulates leaf rolling in rice
Yang Xu1,2,3, Weiyi Kong4, Fangquan Wang1,2,3
1Institute of Food Crops, Jiangsu Academy of Agricultural Sciences, Nanjing, China.
Plant Biotechnology Journal
|August 27, 2021
Summary
Rice leaf rolling is influenced by ROC8 and ROC5 transcription factors. Their heterodimer, not homodimers, is crucial for regulating leaf architecture by affecting bulliform cell development.
Area of Science:
- Plant Biology
- Genetics
- Molecular Biology
Background:
- Moderately rolled leaf is a key trait for ideal rice plant architecture.
- Genes like ROC5 and ROC8 (homeodomain leucine zipper IV transcription factors) regulate rice leaf rolling, but their molecular mechanisms are unclear.
Purpose of the Study:
- To elucidate the molecular mechanism of ROC5 and ROC8 in regulating rice leaf rolling.
- To investigate the role of ROC5 and ROC8 dimerization in leaf development.
Main Methods:
- Demonstrated transcription activation activity of ROC5 and ROC8.
- Analyzed phenotypes of ROC8 overexpression and knockout lines.
- Investigated homodimer and heterodimer formation between ROC5 and ROC8.
- Conducted gene expression analysis in single and double mutants.
Main Results:
- Overexpression of ROC8 led to adaxially rolled leaves by decreasing bulliform cell number and size.
- ROC8 knockout resulted in abaxially rolled leaves due to increased bulliform cells.
- ROC8 and ROC5 form a functional heterodimer, essential for normal leaf rolling.
- ROC8 and ROC5 exhibit additive effects, with double mutants showing stronger phenotypes.
Conclusions:
- The ROC8-ROC5 heterodimer plays a critical role in regulating rice leaf rolling.
- Dimerization of ROC transcription factors is a key mechanism controlling leaf development in rice.
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