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Updated: Oct 22, 2025

Measuring In Vitro ATPase Activity for Enzymatic Characterization
Published on: August 23, 2016
New wine in old bottles: current progress on P5 ATPases
Zhiwen Huang1, Zhigang Feng1, Yan Zou1
1School of Life Science and Technology, ShanghaiTech University, China.
Abstract:
P5 ATPases are evolutionarily conserved P-type transporters. Despite their important roles in the endoplasmic reticulum (ER) and in lysosomes, the substrate specificities and transporting mechanisms of P5 ATPases have remained mysterious. Recently, several studies have provided genetic, biochemical, and structural evidence to help elucidate the physiological functions and substrates of P5 ATPases. Here, we summarize this progress and discuss the potential transport mechanisms of the P5 ATPases-in particular, P5A ATPase-for further study.
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