Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

In-vitro Mutagenesis

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A coordinated transcriptional program controls de novo Golgi biogenesis.

The EMBO journal·2026

Same author

Ultrastructure, Polarity, and Reproduction of the Golgi Apparatus.

Biology of the cell·2026

Same author

The Functions of RAB GTPases at the Golgi Complex.

Sub-cellular biochemistry·2026

Same author

[When physics meets biology].

Medecine sciences : M/S·2025

Same author

Homage to Michel Bornens, who passed away on March 9, 2022 at the age of 84.

EMBO reports·2024

Same author

The GTPase RAB6 is required for stem cell maintenance and cell migration in the gut epithelium.

Development (Cambridge, England)·2024

Same journal

Isolation of Mesenchymal Stem Cell-Derived Extracellular Vesicles.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Modeling Melanoma Immune Surveillance by CAR-T Cells in Human Skin Organoids.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Stepwise Optimization of a Matrigel-Based In Vitro Angiogenesis Assay for Reproducible and Quantifiable 2D-Tube Formation Using HUVECs.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Quantifying Mechanical Properties of Fresh Ovarian Tissue with Optical Brillouin Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

3D Chromatin Architecture During Early Development: New Methods and New Findings.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Metabolic Plasticity in Embryogenesis Throughout the Lens of NAD<sup></sup>.

Methods in molecular biology (Clifton, N.J.)·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Oct 22, 2025

Generation of Maternal Mutants Using zpc:cas9 Knock-in Zebrafish

Generation of Maternal Mutants Using zpc:cas9 Knock-in Zebrafish

Published on: July 22, 2025

Generating Rab6 Conditional Knockout Mice.

Sabine Bardin¹, Bruno Goud²

¹Molecular Mechanisms of Intracellular Transport, Institut Curie, PSL Research University, Centre National de la Recherche Scientifique, UMR 144, Paris, France.

Methods in Molecular Biology (Clifton, N.J.)

|August 28, 2021

Summary

This summary is machine-generated.

Researchers created conditional knockout mice to study RAB6 GTPase function. This overcomes the embryonic lethality of full Rab6a knockout, enabling tissue-specific analysis of this vital Golgi-associated protein.

Keywords:

Cre/lox system Golgi RAB GTPases knockout (k/o) mice

More Related Videos

Generation of Brown Fat-Specific Knockout Mice Using a Combined Cre-LoxP, CRISPR-Cas9, and Adeno-Associated Virus Single-Guide RNA System

Generation of Brown Fat-Specific Knockout Mice Using a Combined Cre-LoxP, CRISPR-Cas9, and Adeno-Associated Virus Single-Guide RNA System

Published on: March 24, 2023

Removal of an Internal Translational Start Site from mRNA While Retaining Expression of the Full-Length Protein

Removal of an Internal Translational Start Site from mRNA While Retaining Expression of the Full-Length Protein

Published on: March 16, 2022

Related Experiment Videos

Last Updated: Oct 22, 2025

Generation of Maternal Mutants Using zpc:cas9 Knock-in Zebrafish

Generation of Maternal Mutants Using zpc:cas9 Knock-in Zebrafish

Published on: July 22, 2025

Generation of Brown Fat-Specific Knockout Mice Using a Combined Cre-LoxP, CRISPR-Cas9, and Adeno-Associated Virus Single-Guide RNA System

Generation of Brown Fat-Specific Knockout Mice Using a Combined Cre-LoxP, CRISPR-Cas9, and Adeno-Associated Virus Single-Guide RNA System

Published on: March 24, 2023

Removal of an Internal Translational Start Site from mRNA While Retaining Expression of the Full-Length Protein

Removal of an Internal Translational Start Site from mRNA While Retaining Expression of the Full-Length Protein

Published on: March 16, 2022

Area of Science:

Cell Biology
Molecular Biology
Genetics

Background:

RAB6 GTPase is a key regulator of intracellular transport at the Golgi apparatus.
Complete knockout of Rab6a in mice results in embryonic lethality, hindering functional studies in specific tissues.
The Cre/lox system offers a method for conditional gene deletion.

Purpose of the Study:

To investigate the function of RAB6 GTPase in specific cell lineages and tissues.
To overcome the limitations imposed by embryonic lethality of Rab6a knockout mice.
To develop tools for dissecting RAB6A's role in cellular processes.

Main Methods:

Generation of conditional Rab6a knockout mouse models using the Cre/lox recombination system.
Utilizing Cre recombinase to induce deletion of the Rab6a gene in a tissue-specific or temporal-controlled manner.
Analysis of cellular and physiological consequences of RAB6A depletion.

Main Results:

Conditional Rab6a knockout mice were successfully generated, allowing for viable offspring.
These models permit the study of RAB6A function in various cell types and developmental stages.
Initial studies can now explore RAB6A's role in Golgi transport within specific contexts.

Conclusions:

Conditional knockout strategies are essential for studying essential genes with embryonic lethal phenotypes.
The generated Rab6a conditional knockout mice provide valuable models for understanding RAB6A's diverse functions.
Further research using these models will elucidate RAB6A's precise roles in organelle transport and cellular homeostasis.