Dual-mode spectrum of transmission and fluorescence using single ultraviolet LED light source and their application...

In order to improve the accuracy of spectral analysis of complex solutions, based on the "M + N" theory, this paper proposes to use single (365 nm ± 5 nm) ultraviolet LED as emission light to detect transmission spectrum and excited fluorescence spectrum. Taking the total bilirubin in serum as the measurement object, the dual-mode spectrum of transmission and fluorescence about serum is collected, which increases the amount of information. In order to verify the effectiveness of the method proposed in this paper, the transmission and fluorescence spectra of the samples were also collected. Then three models of total bilirubin concentration are established by transmission spectrum, fluorescence spectrum and dual-mode spectrum of transmission and fluorescence respectively. Through the comparison of the parameters of the three models, the model established by dual-mode spectrum of transmission and fluorescence is good. The Rc of the model is 0.91 and the RMSEC is 3.00 (μmol/L). The Rp is 0.92, the RMSEP is 3.53 (μmol/L). Compared with transmission spectrum modeling and fluorescence spectrum modeling, the RMSEP of dual-mode spectrum modeling was reduced by 34.8% and 22.6% respectively. The experimental results show that the measurement method of dual-mode spectrum of transmission and fluorescence by using a single ultraviolet light source proposed in this paper based on the "M + N" theory increases the information of solution composition, which provides a new method for the analysis of the same characteristic components.