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Related Experiment Video

Updated: Oct 22, 2025

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A decoy strategy to activate the immune system.

Manisha S Deshpande1, Tanushree Banerjee1,2

  • 1Dr. D.Y. Patil Biotechnology and Bioinformatics Institute, Dr. D.Y. Patil Vidyapeeth, Pune, Maharashtra, India.

IUBMB Life
|August 29, 2021
PubMed
Summary

A novel vaccine combines a fusion protein with inactivated SARS-CoV-2 to enhance immune response. This platform strategy offers a promising approach for developing effective vaccines and therapeutics against viral infections.

Keywords:
HBsAgN-terminal helixSARS-CoV-2decoyfusion proteinimmune activation

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Area of Science:

  • Vaccinology
  • Virology
  • Immunology

Background:

  • SARS-CoV-2 utilizes the ACE2 receptor for cellular entry.
  • Existing vaccines and therapeutic strategies face challenges in efficacy and broad applicability.

Purpose of the Study:

  • To present a novel two-component vaccine strategy using a fusion protein and inactivated virus.
  • To explore the therapeutic potential of the fusion protein in treating existing viral infections.

Main Methods:

  • Development of a fusion protein: Hepatitis B surface antigen (HBsAg) conjugated to the N-terminal helix (NTH) of ACE2.
  • Administration of the fusion protein with whole inactivated SARS-CoV-2 for vaccination.
  • Administration of the fusion protein alone for therapeutic purposes.

Main Results:

  • The fusion protein's NTH binds to the SARS-CoV-2 Spike protein's RBD.
  • HBsAg acts as a decoy, enhancing immune presentation and antibody response.
  • Potential for neutralization of infecting viruses through immune system harnessing.

Conclusions:

  • The fusion protein-inactivated virus vaccine platform offers enhanced efficacy and antigen presentation.
  • This platform strategy is adaptable for future viral threats.
  • The fusion protein demonstrates potential as a therapeutic agent for viral infections.