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Updated: Oct 22, 2025

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CircRNA DUSP16 Knockdown Suppresses Colorectal Cancer Progression by Regulating the miR-432-5p/E2F6 Axis

Guangyao Wang1, Haojun Yang1

  • 1Department of Gastrointestinal Surgery, The Affiliated Changzhou No.2 People's Hospital of Nanjing Medical University, Changzhou, 213000, People's Republic of China.

Cancer Management and Research
|August 30, 2021
PubMed
Abstract

Insights

Circular RNA DUSP16 (circDUSP16) promotes colorectal cancer (CRC) by upregulating E2F6 via sponging miR-432-5p. Silencing circDUSP16 inhibits CRC progression and tumor growth, offering a potential therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Circular RNAs (circRNAs) are implicated in various cancers.
  • The role of circRNA DUSP16 (circDUSP16) in colorectal cancer (CRC) remains largely unexplored.

Purpose of the Study:

  • To investigate the function of circDUSP16 in colorectal cancer development.
  • To elucidate the underlying molecular mechanism of circDUSP16 in CRC.

Main Methods:

  • Quantitative real-time PCR (RT-qPCR) for gene expression analysis.
  • Cellular assays (CCK-8, Transwell, flow cytometry) for proliferation, migration, invasion, and apoptosis.
  • Western blot for apoptosis-related proteins.
  • Luciferase reporter and RNA immunoprecipitation (RIP) assays for molecular interactions.
  • In vivo xenograft tumor model to assess tumor growth.

Main Results:

  • CircDUSP16 was upregulated in CRC tissues and cell lines, correlating with poor survival.
  • Knockdown of circDUSP16 suppressed CRC cell proliferation, migration, invasion, and induced apoptosis.
  • CircDUSP16 positively regulated E2F6 expression by negatively regulating miR-432-5p.
  • CircDUSP16 silencing repressed CRC tumor growth in vivo.

Conclusions:

  • CircDUSP16 knockdown suppresses CRC progression by modulating the miR-432-5p/E2F6 axis.
  • The circDUSP16/miR-432-5p/E2F6 network represents a potential therapeutic target for colorectal cancer.
Keywords:
E2F6circDUSP16colorectal cancermiR-432-5pprogression

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