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Related Concept Videos

UV–Vis Spectrometers01:14

UV–Vis Spectrometers

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The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell.
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Spectrophotometry: Introduction01:16

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Spectrophotometry is the quantitative measurement of the absorption, reflection, diffraction, or transmission of electromagnetic radiation through a material as a function of the intensity and wavelength of the radiation. A spectrophotometer is a device used to measure the change in the radiation intensity caused by its interaction with the material.
The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...
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Related Experiment Video

Updated: Oct 22, 2025

Rapid Viscoelastic Characterization of Airway Mucus Using a Benchtop Rheometer
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Reflectance spectra analysis for mucous assessment.

Toru Chiba1, Masaharu Murata2, Takahito Kawano2

  • 1Pentax_LifeCare, HOYA Corporation, Akishima-shi 196-0012, Tokyo, Japan. toru.chiba@pentaxmedical.com.

World Journal of Gastrointestinal Oncology
|August 30, 2021
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Summary

Medical hyperspectral imaging offers advanced disease diagnosis and surgical guidance. This technology maps blood oxygen saturation (SatO2) and tissue properties, aiding in detecting gastrointestinal disorders and tumors.

Keywords:
Diffuse reflectance spectroscopyEndoscopyGastrointestinal cancerHemoglobin saturationHypoxiaTumor

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Area of Science:

  • Medical Imaging
  • Biomedical Optics
  • Spectroscopy

Background:

  • Spectral imaging is emerging as a novel modality for medical applications.
  • It is particularly relevant for disease diagnosis and image-guided surgery.
  • Advances enable detailed physiological and morphological tissue analysis.

Purpose of the Study:

  • To review research and development in medical hyperspectral imaging.
  • To highlight its applications in disease diagnosis and surgical guidance.
  • To discuss the potential of spectral imaging for tissue analysis.

Main Methods:

  • Review of existing research and development in medical hyperspectral imaging.
  • Examination of analytical algorithms for spectral data processing.
  • Focus on two-dimensional mapping of blood oxygen saturation (SatO2) and hemoglobin concentration.

Main Results:

  • Hyperspectral imaging provides accurate, high-resolution mapping of SatO2 and hemoglobin concentration.
  • The technology offers insights into tissue oxygenation, perfusion, and function.
  • Studies indicate a link between blood flow, SatO2, and gastrointestinal disorders, including malignancy.

Conclusions:

  • Medical hyperspectral imaging shows significant promise for practical biomedical applications.
  • It offers valuable diagnostic information for tissue physiology and morphology.
  • Spectroscopic image analysis may play a key role in detecting and diagnosing diseases like gastrointestinal tumors.