Related Experiment Video

Updated: Oct 22, 2025

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope
08:09

Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope

Published on: March 24, 2017

9.7K

Linear and Continuous Flavivirus Epitopes From Naturally Infected Humans

Marcilio Jorge Fumagalli1, Luiz Tadeu Moraes Figueiredo1, Victor Hugo Aquino2

  • 1Virology Research Center, Medical School of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil.

Frontiers in Cellular and Infection Microbiology
|August 30, 2021
PubMed

Insights

This review maps 153 validated linear and continuous epitopes from five flaviviruses, including Dengue virus (DENV). This data aids understanding antibody responses and developing vaccines and therapies against these arboviruses.

Area of Science:

  • Virology and Immunology
  • Epitope Mapping
  • Arbovirus Research

Background:

  • Flaviviruses, transmitted by arthropods, pose significant global health threats, necessitating a deeper understanding of host immune responses.
  • Effective vaccines and therapeutics for many flavivirus infections are lacking, highlighting the need for detailed immunological data.
  • Epitopes, the specific sites recognized by antibodies, are crucial for diagnostics, vaccine design, and immunotherapy.

Purpose of the Study:

  • To consolidate and review experimentally validated linear and continuous epitopes from key flavivirus species.
  • To analyze the characteristics and potential applications of these identified epitopes.
  • To provide a comprehensive resource for researchers studying flavivirus immunity.

Main Methods:

  • Systematic literature review of studies published between 1989 and 2020.
  • Inclusion of peptides from structural and nonstructural proteins of Dengue virus, Zika virus, Japanese encephalitis virus, West Nile virus, and tick-borne encephalitis virus.
  • Focus on peptides demonstrating immunoreactivity with human antibodies from natural infections or vaccinations.

Main Results:

  • Compilation of 153 immunoreactive peptides from five major flaviviruses.
  • Majority of epitopes identified for Dengue virus (DENV), reflecting its public health burden.
  • Epitopes were derived from both structural (envelope, capsid, pre-membrane) and nonstructural (NS1-5) viral proteins.

Conclusions:

  • The identified epitopes offer valuable insights into the antibody landscape of flavivirus infections.
  • These data are critical for developing improved diagnostic tools, vaccines, and immunotherapies against flaviviruses.
  • Further research is needed to fully elucidate the immune landscape of linear and continuous flavivirus epitopes.
Keywords:
arbovirusflavivirushuman antibodiesimmunoreactive peptideslinear epitopes

More Related Videos

Detection of Neutralization-sensitive Epitopes in Antigens Displayed on Virus-Like Particle VLP-Based Vaccines Using a Capture Assay05:15

Detection of Neutralization-sensitive Epitopes in Antigens Displayed on Virus-Like Particle VLP-Based Vaccines Using a Capture Assay

Published on: February 10, 2022

3.8K
Zika Virus Specific Diagnostic Epitope Discovery11:37

Zika Virus Specific Diagnostic Epitope Discovery

Published on: December 12, 2017

8.0K

Related Concept Videos

Viruses with RNA Genomes01:29

Viruses with RNA Genomes

RNA viruses are categorized into positive-strand, negative-strand, or double-stranded groups based on their genomic structure and replication mechanisms. This classification dictates how they exploit host cellular machinery for protein synthesis and replication. Some RNA viruses also utilize reverse transcription as part of their life cycle, further diversifying their replication strategies.Positive-Strand RNA VirusesPositive-strand RNA viruses have genomes that function directly as messenger...
255
Leaky Scanning02:28

Leaky Scanning

During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R...
5.3K
Viral Recombination00:57

Viral Recombination

Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.
24.0K