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Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope
Published on: March 24, 2017
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Linear and Continuous Flavivirus Epitopes From Naturally Infected Humans
Marcilio Jorge Fumagalli1, Luiz Tadeu Moraes Figueiredo1, Victor Hugo Aquino2
1Virology Research Center, Medical School of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil.
Frontiers in Cellular and Infection Microbiology
|August 30, 2021
Summary
This review maps 153 validated linear and continuous epitopes from five flaviviruses, including Dengue virus (DENV). This data aids understanding antibody responses and developing vaccines and therapies against these arboviruses.
Area of Science:
- Virology and Immunology
- Epitope Mapping
- Arbovirus Research
Background:
- Flaviviruses, transmitted by arthropods, pose significant global health threats, necessitating a deeper understanding of host immune responses.
- Effective vaccines and therapeutics for many flavivirus infections are lacking, highlighting the need for detailed immunological data.
- Epitopes, the specific sites recognized by antibodies, are crucial for diagnostics, vaccine design, and immunotherapy.
Purpose of the Study:
- To consolidate and review experimentally validated linear and continuous epitopes from key flavivirus species.
- To analyze the characteristics and potential applications of these identified epitopes.
- To provide a comprehensive resource for researchers studying flavivirus immunity.
Main Methods:
- Systematic literature review of studies published between 1989 and 2020.
- Inclusion of peptides from structural and nonstructural proteins of Dengue virus, Zika virus, Japanese encephalitis virus, West Nile virus, and tick-borne encephalitis virus.
- Focus on peptides demonstrating immunoreactivity with human antibodies from natural infections or vaccinations.
Main Results:
- Compilation of 153 immunoreactive peptides from five major flaviviruses.
- Majority of epitopes identified for Dengue virus (DENV), reflecting its public health burden.
- Epitopes were derived from both structural (envelope, capsid, pre-membrane) and nonstructural (NS1-5) viral proteins.
Conclusions:
- The identified epitopes offer valuable insights into the antibody landscape of flavivirus infections.
- These data are critical for developing improved diagnostic tools, vaccines, and immunotherapies against flaviviruses.
- Further research is needed to fully elucidate the immune landscape of linear and continuous flavivirus epitopes.
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