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Updated: Oct 22, 2025

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Studying RNA Interactors of Protein Kinase RNA-Activated during the Mammalian Cell Cycle
Published on: March 5, 2019
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Measurement of RNA-induced PKR Activation in vitro
Kobe C Yuen1,2
1Stowers Institute for Medical Research, Kansas City, MO, USA.
Bio-Protocol
|August 30, 2021
Abstract:
Protein kinase R (PKR) is one of the key RNA-activated sensors for innate immunity. PKR is activated by pathogenic or aberrant RNAs such as short double-stranded RNAs or those with imperfect secondary structures, as well as a reduction in the amount and number of RNA modifications. Activation of PKR may be an underlying mechanism for the pathogenesis of human diseases. In this protocol, I describe a method for studying levels of RNA-induced PKR activation in vitro.

