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Updated: Oct 22, 2025
Click-Chemistry Based Fluorometric Assay for Apolipoprotein N-acyltransferase from Enzyme Characterization to High-Throughput Screening
Published on: May 13, 2020
One-pot fluorescent assay for sensitive detection of APOBEC3A activity
Bingyao Wang1, Xiong Zhang1, Yafen Wang1
1The Institute of Advanced Studies, College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of Ministry of Education, Hubei Province Key Laboratory of Allergy and Immunology, Wuhan University Wuhan Hubei 430072 China xzhou@whu.edu.cn xcweng@whu.edu.cn +86-27-68756663 +86-27-68756663.
Abstract:
We reported a one-pot fluorescence-based assay to quantitively detect A3A activity combined with cytosine deamination and uracil excision. After deamination by A3A and USER enzyme treatment, the fluorescent turn-on effect at 520 nm was observed, which can be used to evaluate the A3A activity and screen inhibitors.
