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Related Experiment Video

Updated: Oct 22, 2025

Screening for Thermotoga maritima Membrane-Bound Pyrophosphatase Inhibitors
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Exploration of Pyrazolo[1,5-a]pyrimidines as Membrane-Bound Pyrophosphatase Inhibitors.

Niklas G Johansson1, Loïc Dreano1, Keni Vidilaseris2

  • 1Drug Research Program, Division of Pharmaceutical Chemistry and Technology, Faculty of Pharmacy, University of Helsinki, P.O. Box 56 (Viikinkaari 5 E), 00014, Helsinki, Finland.

Chemmedchem
|August 30, 2021
PubMed
Summary

New small molecules targeting membrane-bound pyrophosphatase (mPPase) show promise against parasitic protozoa. Researchers identified novel pyrazolo[1,5-a]pyrimidine compounds that inhibit mPPase and Plasmodium falciparum growth.

Keywords:
antiprotozoal agentsdrug discoveryinhibitormembrane-bound pyrophosphatasepyrazolo[1,5-a]pyrimidine

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Area of Science:

  • Biochemistry
  • Medicinal Chemistry
  • Parasitology

Background:

  • Membrane-bound pyrophosphatase (mPPase) is essential for protist survival but absent in humans.
  • mPPases couple pyrophosphate hydrolysis to ion transport across acidocalcisomal membranes.
  • Few non-phosphorus inhibitors of mPPase have been reported.

Purpose of the Study:

  • To explore chemical space for novel mPPase inhibitors.
  • To identify and characterize new scaffolds with mPPase inhibitory activity.
  • To evaluate the anti-parasitic potential of identified compounds.

Main Methods:

  • Screening and synthetic medicinal chemistry approaches were employed.
  • Compounds were tested against Thermotoga maritima mPPase.
  • Structure-activity relationships were established for a pyrazolo[1,5-a]pyrimidine scaffold.

Main Results:

  • Novel pyrazolo[1,5-a]pyrimidine compounds with low micromolar inhibitory activity against mPPase were identified.
  • Early structure-activity relationships were determined for the new scaffold.
  • The lead compound inhibited Plasmodium falciparum mPPase and ex vivo parasite growth.

Conclusions:

  • Small molecule inhibition of mPPase represents a viable strategy against pathogenic protozoa.
  • Pyrazolo[1,5-a]pyrimidine derivatives are promising candidates for further drug development.
  • Targeting mPPase could offer a new therapeutic avenue for malaria and other parasitic diseases.