In vivo biocompatibility of diamond-like carbon films containing TiO₂ nanoparticles for biomedical applications

Hybrid diamond-like carbon (DLC) with incorporated titanium dioxide (TiO₂) nanoparticle coatings have low friction coefficient, high wear resistance, high hardness, biocompatibility, and high chemical stability. They could be employed to modify biomedical alloys surfaces for numerous applications in biomedical engineering. Here we investigate for the first time the in vivo inflammatory process of DLC coatings with incorporated TiO₂ nanoparticles. TiO₂-DLC films were grown on AISI 316 stainless-steel substrates using plasma-enhanced chemical vapor deposition. The coated substrates were implanted in CF1 mice peritoneum. The in vivo cytotoxicity and biocompatibility of the samples were analyzed from macrophage lavage. Analysis in the first weeks after implantation could be helpful to evaluate the acute cytotoxicity generated after a possible inflammatory process. The in vivo results showed no inflammatory process. A significant increase in nitric oxide production on the uncoated substrates was confirmed through cytometry, and the coated substrates demonstrated biocompatibility. The presence of TiO₂ nanoparticles enhanced the wound healing activity, due to their astringent and antimicrobial properties. DLC and TiO₂-DLC coatings were considered biocompatible, and the presence of TiO₂ nanoparticles reduced the inflammatory reactions, increasing DLC biocompatibility.