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Related Concept Videos

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The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte...
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Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
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Multi-analyte Biochip MAB Based on All-solid-state Ion-selective Electrodes ASSISE for Physiological Research
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Multi-Analyte Detection Based on Integrated Internal and External Sensing Approach.

Firoz Haider, Md Mashrafi, Rifat Ahmmed Aoni

    IEEE Transactions on Nanobioscience
    |August 30, 2021
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    Summary

    This study introduces a novel photonic crystal fiber sensor for highly sensitive, simultaneous detection of multiple analytes. This advancement supports rapid point-of-care diagnosis of various biomarkers.

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    Area of Science:

    • Photonics
    • Nanotechnology
    • Biomedical Engineering

    Background:

    • Point-of-care (POC) diagnostics require sensitive, simple, and multiplex detection for clinical samples.
    • Existing methods may lack the sensitivity or multiplexing capability for comprehensive POC analysis.

    Purpose of the Study:

    • To propose a novel photonic crystal fiber (PCF) based surface plasmon resonance (SPR) sensor for simultaneous detection of multiple analytes.
    • To achieve high sensitivity and resolution for diverse biological targets at the POC platform.

    Main Methods:

    • Utilizing a PCF-based SPR sensor with internal and external sensing approaches.
    • Employing scaled-down air-holes to excite plasmonic metal layers for light guiding.
    • Numerical analysis of light-guiding and sensing properties using the Finite Element Method (FEM).

    Main Results:

    • Achieved maximum wavelength sensitivities of 12,000 nm/RIU (internal) and 10,000 nm/RIU (external).
    • Obtained resolutions of 8.33×10^-6 RIU and 1.0×10^-5 RIU for internal and external sensing, respectively.
    • Demonstrated applicability for detecting analytes with refractive indices from 1.33 to 1.40, including viruses, cancer cells, glucose, proteins, and DNA/RNA.

    Conclusions:

    • The proposed hybrid sensor offers high sensitivity and multiplexing capability for POC applications.
    • This technology can significantly advance the detection of various bio-targets in clinical settings.
    • The sensor's performance supports its role in developing next-generation diagnostic tools.