A concise detection strategy of Staphylococcus aureus using N-Succinyl-Chitosan-dopped bacteria-imprinted composite...

Staphylococcus aureus is one of the major foodborne pathogens. Efficient detection and isolation of Staphylococcus aureus from complex samples are crucial. Herein, we report a concise strategy to detect of Staphylococcus aureus with high sensitivity and specificity, based on N-Succinyl-Chitosan doping bacteria-imprinted composite film and aggregation-induced emission (AIE)-featuring fluorescence sensor. The good shaping and mechanical properties of polydimethylsiloxane provide a specific recognition site suitable for Staphylococcus aureus. For the first time, chitosan derivatives is combined with polydimethylsiloxane to prepare a two-component composite film, which possesses a remarkable absorption performance of Staphylococcus aureus using the natural excellent absorption property of chitosan. The positive charged AIE-featuring Au(I)-disulfide nanoparticles realized the quantitative characterization of Staphylococcus aureus without cooperation with bio-recognition elements. To conclude, this study provides new possibilities for the manufacture of highly efficient bacterial separators with superior performance and facilitates the application of unlabeled nanoparticles in quantitative analysis.