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Updated: Oct 22, 2025
Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection
Published on: June 5, 2021
A tissue culture infectious dose-derived protocol for testing of SARS-CoV-2 neutralization of serum antibodies on
Fabio Hasler1, Agathe Duda2, Thomas M Kündig1,2
1Department of Dermatology, University of Zurich, 8091 Zurich, Switzerland.
Abstract:
For a cytopathic virus such as severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2), the neutralization capacity of serum from convalescent or vaccinated persons or of therapeutic antibodies can be tested on adherent cell cultures. Here, a simple and tissue culture infectious dose-derived protocol for assessment of neutralization of SARS-CoV-2 is described. Compared with the often applied plaque-forming unit assay, the working load is lower, and fewer manipulations of the infected cultures are required. Hence, the method is safer for the personnel.
