General Methods to Produce and Assemble Recombinant Spider Silk Proteins

Orb-weaving spiders are known to spin up to seven types of silks/glues from different silk glands. The inherent mechanical variety of these silks makes them attractive models for a variety of biomaterial design, from superglues to extremely strong and/or extendible fibers. Spider silk spinning is a process in which spinning dope stored in specific glands assembles into fibrils upon chemical and mechanical stimuli. The exploration of silk protein assembly into controllable filaments is vital for both uncovering biological functions and molecular structure relationship, as well as fabricating new biomaterials. This chapter describes the methods for biosynthesis and assembly of recombinant spider silk proteins, which will provide insights into the mechanism exploration of fiber formation and spider silk-based material manufacture.