Self-Assembly of Bombyx mori Silk Fibroin

Silk fibroin from Bombyx mori (silkworm) distinguishes for its unique mechanical performance, controllable degradation rates, and easily large-scale production, making it attractive models for a variety of biomaterial design. These outstanding properties of silk fibroin originate from its unique modular composition of silk proteins. To exploit the structure-function relationship and fabricate silk fibroin-based biomaterials, comprehensive strategies to uncover assembly behaviors of fibrous proteins are necessary. This chapter describes methods to produce regenerated silk fibroin protein from Bombyx mori silk and their self-assembly strategies. This could provide insight into the fabrication of various silk fibroin-based biomaterials, such as hydrogels, tubes, sponges, fibers, microspheres, and diverse thin film patterns, which can be used for textiles, electronics and optics, environmental engineering, and biomedical applications.