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ISFET based enzyme sensors.

B H van der Schoot1, P Bergveld

  • 1Department of Electrical Engineering, University of Twente, Enschede, The Netherlands.

Biosensors
|January 1, 1987
PubMed
Summary
This summary is machine-generated.

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This review covers ISFET-based enzyme sensors, highlighting fabrication improvements over glass electrodes. A novel coulometric system addresses pH dependence and buffer capacity issues, enabling a pH-static sensor with expanded linear range.

Area of Science:

  • Electrochemistry
  • Biosensors
  • Enzyme technology

Background:

  • Ion-selective field-effect transistors (ISFETs) offer fabrication advantages for enzyme sensors compared to traditional glass electrodes.
  • Existing ISFET-based enzyme sensors face challenges including pH dependence, limited dynamic range, and sensitivity to sample buffer capacity.

Purpose of the Study:

  • To review the reported results of ISFET-based enzyme sensors.
  • To introduce a novel coulometric system to overcome limitations in current ISFET enzyme sensor technology.

Main Methods:

  • Review of existing literature on ISFET-based enzyme sensors.
  • Development and introduction of a coulometric system for pH control within the immobilized enzyme layer.

Main Results:

Related Experiment Videos

  • ISFETs provide significant improvements in sensor fabrication methods.
  • The proposed coulometric system effectively compensates for analyte buffer capacity.
  • The developed pH-static enzyme sensor demonstrates independence from sample pH and buffer capacity, alongside an expanded linear range.

Conclusions:

  • The novel coulometric system offers a viable solution to critical challenges in ISFET enzyme sensor design.
  • pH-static enzyme sensors present a promising advancement for reliable and robust biosensing applications.