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Updated: Oct 21, 2025

Conducting Multiple Imaging Modes with One Fluorescence Microscope
Published on: October 28, 2018
Fluorescence imaging with tailored light
Jialei Tang1,2, Jinhan Ren1,2, Kyu Young Han1
1CREOL, The College of Optics and Photonics, University of Central Florida, Orlando, Florida, USA.
Abstract:
Fluorescence microscopy has long been a valuable tool for biological and medical imaging. Control of optical parameters such as the amplitude, phase, polarization and propagation angle of light gives fluorescence imaging great capabilities ranging from super-resolution imaging to long-term real-time observation of living organisms. In this review, we discuss current fluorescence imaging techniques in terms of the use of tailored or structured light for the sample illumination and fluorescence detection, providing a clear overview of their working principles and capabilities.
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