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Updated: Oct 21, 2025
11:16
Correlative Light- and Electron Microscopy Using Quantum Dot Nanoparticles
Published on: August 7, 2016
9.9K
Correlative light and volume electron microscopy (vCLEM): How community participation can advance developing
Christopher J Guerin1, Saskia Lippens1
1VIB Bio Imaging Core, VIB - Ghent University, Ghent, Belgium.
Journal of Microscopy
|September 3, 2021
Summary
Correlative light and electron microscopy (CLEM) advances imaging by linking structural and functional data across scales. Recent innovations make this powerful technique accessible for large-scale biological research.
Area of Science:
- Cell Biology
- Microscopy
- Biotechnology
Background:
- Correlative light and electron microscopy (CLEM) integrates data across resolution scales.
- Historically a niche technique, CLEM has seen significant advancements since the 1960s.
- Recent developments have expanded CLEM's applicability to large biological samples.
Purpose of the Study:
- To highlight the evolution and mainstreaming of CLEM techniques.
- To emphasize the role of collaboration in advancing imaging technologies.
- To showcase the impact of imaging pioneers on scientific accessibility.
Main Methods:
- Utilizes correlative light and electron microscopy workflows.
- Incorporates advanced image processing technologies.
- Facilitates integration of structural and functional data.
Main Results:
- Recent developments enable CLEM application to large volumes of cells and tissues.
- Collaborative efforts have successfully integrated methods, hardware, and software.
- CLEM is transitioning from a niche area to a mainstream microscopy technique.
Conclusions:
- CLEM is a valuable tool for multi-resolution imaging and data integration.
- Collaborations between researchers and industry are crucial for technological advancement.
- Pioneering efforts have made sophisticated imaging techniques more widely available.
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