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Updated: Oct 21, 2025

Nanosensors to Detect Protease Activity In Vivo for Noninvasive Diagnostics

Nanosensors to Detect Protease Activity In Vivo for Noninvasive Diagnostics

Published on: July 16, 2018

Simultaneous detection of multiple proteases using a non-array nanopore platform.

Xiaohan Chen¹, Youwen Zhang, Xiyun Guan

¹Department of Chemistry, Illinois Institute of Technology, 3101 S Dearborn St, Chicago, IL 60616, USA. xguan5@iit.edu.

|September 3, 2021

Summary

This summary is machine-generated.

This study introduces a novel nanopore sensing platform for simultaneously measuring multiple proteases, specifically ADAM10 and ADAM17, in a single assay. This non-array method offers a new approach for multiplexed analyte detection and clinical diagnostics.

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Last Updated: Oct 21, 2025

Nanosensors to Detect Protease Activity In Vivo for Noninvasive Diagnostics

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Published on: July 16, 2018

Author Spotlight: Engineering Molecular Tools for Disease Detection and Imaging

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Visual Detection of Multiple Nucleic Acids in a Capillary Array

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

Area of Science:

Biotechnology
Analytical Chemistry
Biochemistry

Background:

Multiplexing assays are crucial for measuring multiple analytes simultaneously.
Current methods often rely on sensor arrays, limiting scalability.
Protease activity is vital in various biological processes and disease states.

Purpose of the Study:

To develop an innovative multiplex multi-analyte detection platform for protease measurement.
To enable simultaneous detection and quantification of ADAM10 and ADAM17 in mixture samples.
To explore a non-array format for enhanced multiplexing capabilities.

Main Methods:

Utilized a single peptide substrate with two distinct cleavage sites for ADAM10 and ADAM17.
Employed a single nanopore to monitor the degradation of the peptide substrate.
Quantified protease activity by analyzing the degradation products in real-time.

Main Results:

Successfully achieved simultaneous detection and quantification of ADAM10 and ADAM17 in mixed samples.
Demonstrated the efficacy of the non-array multiplexing sensing platform.
Validated the platform's potential for sensitive and specific protease measurement.

Conclusions:

The developed platform offers a novel approach for multiplexed protease detection.
This non-array sensing strategy can be integrated with existing sensor arrays to enhance multiplexing capacity.
The technology holds promise for applications in clinical diagnosis and prognosis.