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Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

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Related Experiment Video

Updated: Oct 21, 2025

Spatiotemporally Controlled Nuclear Translocation of Guests in Living Cells Using Caged Molecular Glues as Photoactivatable Tags

Spatiotemporally Controlled Nuclear Translocation of Guests in Living Cells Using Caged Molecular Glues as Photoactivatable Tags

Published on: January 17, 2019

Publisher Correction: Live cell tagging tracking and isolation for spatial transcriptomics using photoactivatable

Alex S Genshaft^1,2,3,4,5,6, Carly G K Ziegler^1,2,3,4,5,6, Constantine N Tzouanas^1,2,3,4,5,6

¹Institute for Medical Engineering & Science, MIT, Cambridge, MA, USA.

Nature Communications

|September 4, 2021

Summary

No abstract available in PubMed .

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