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Biocompatible Graphene Oxide Nanosheets Densely Functionalized with Biologically Active Molecules for Biosensing
Benjamin A E Lehner1, Dominik Benz2, Stanislav A Moshkalev3
1Kavli Institute of Nanoscience, Delft University of Technology, Delft 2629HZ, The Netherlands.
ACS Applied Nano Materials
|September 6, 2021
Summary
We developed a new method to covalently functionalize graphene oxide (GO) for improved biocompatibility and biomolecule coating. This enhances GO
Area of Science:
- Biomedical Engineering
- Materials Science
- Nanotechnology
Background:
- Graphene oxide (GO) offers potential for biomedical applications due to its unique properties.
- Poor biocompatibility and difficult covalent functionalization limit GO's use in biological systems.
- Current methods yield low biomolecular coating and poor integration.
Purpose of the Study:
- To develop a facile and effective method for covalent biofunctionalization of GO.
- To improve biomolecular coating density and heterogeneity across the GO lattice.
- To enhance GO's performance in biosensing applications.
Main Methods:
- Covalent biofunctionalization of GO using colamine (CA) and a poly(ethylene glycol) cross-linker.
- Assessment of biomolecular coating density and heterogeneity.
- Evaluation of nonspecific biomolecule adhesion suppression and biomarker detection sensitivity in a DNA-biosensing assay.
Main Results:
- Achieved vastly improved biomolecular coating density and heterogeneity on GO.
- Demonstrated superior suppression of nonspecific biomolecule adhesion compared to traditional methods.
- Showcased increased biomarker detection sensitivity in DNA biosensing.
Conclusions:
- The optimized biofunctionalization method significantly enhances GO's biocompatibility and coating.
- This approach overcomes limitations of previous GO modification techniques.
- The improved GO is suitable for advanced in situ biomedical applications like biosensors and drug carriers.
