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Genomic analysis revealed conserved acid tolerance mechanisms from native micro-organisms in fermented feed
Lucrecia C Terán1,2,3, Pablo Mortera1,2, Gisela Tubio4
1Laboratorio de Fisiología y Genética de Bacterias Lácticas, Instituto de Biología Molecular y Celular de Rosario (IBR), sede Facultad de Ciencias Bioquímicas y Farmacéuticas (FBioyF), Universidad Nacional de Rosario (UNR), Consejo Nacional de Ciencia y Tecnología (CONICET), Rosario, Argentina.
Journal of Applied Microbiology
|September 6, 2021
Summary
Two microbial strains, Lactobacillus paracasei IBR07 and Kazachstania unispora IBR014, were identified as safe and effective starter cultures for fermented feed production, enhancing animal growth performance.
Area of Science:
- Agricultural Microbiology
- Animal Nutrition
- Food Science
Background:
- Fermented feed is a global practice to enhance farm animal growth.
- Identifying effective microbial cultures is crucial for optimizing fermentation.
Purpose of the Study:
- To isolate and characterize lactic acid bacteria and yeast for fermented feed.
- To evaluate the safety and suitability of candidate microbial strains.
Main Methods:
- Isolation and genomic analysis of Lactobacillus paracasei IBR07 and Kazachstania unispora IBR014.
- Prediction of metabolic pathways related to acid tolerance.
- Assessment of virulence factors and safety using the Galleria mellonella model.
Main Results:
- Lactobacillus paracasei IBR07 and Kazachstania unispora IBR014 were identified from autochthonous microbiota.
- Genomic analysis revealed pathways for acid response and persistence.
- No virulence factors were detected, and safety was confirmed via the Galleria mellonella model.
Conclusions:
- IBR07 (L. paracasei) and IBR014 (K. unispora) are suitable starter cultures for feed fermentation.
- These findings support further research into the benefits of fermented feed for animal production.
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