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Related Experiment Video

Updated: Oct 21, 2025

Author Spotlight: Advanced Single-Molecule Techniques for Investigating Telomeric Protein-DNA Interactions

Author Spotlight: Advanced Single-Molecule Techniques for Investigating Telomeric Protein-DNA Interactions

Published on: August 30, 2024

Telomerase detection using a DNA-PAINT strategy.

Shenfei Zong¹, Xiangyu Ye¹, Junzhu Zong¹

¹Advanced Photonics Center, Southeast University, Nanjing 210096, Jiangsu, People's Republic of China.

|September 6, 2021

Summary

This summary is machine-generated.

We developed a novel DNA-PAINT method for telomerase detection, enabling high-resolution imaging and direct visualization of telomere elongation. This sensitive approach avoids complex procedures and aids cancer diagnosis.

Keywords:

DNA-PAINT in situ visualization super resolution telomerase telomeric repeats

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Last Updated: Oct 21, 2025

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Telomerase Activity in the Various Regions of Mouse Brain: Non-Radioactive Telomerase Repeat Amplification Protocol TRAP Assay

Telomerase Activity in the Various Regions of Mouse Brain: Non-Radioactive Telomerase Repeat Amplification Protocol TRAP Assay

Published on: September 2, 2014

Area of Science:

Biochemistry
Molecular Biology
Nanotechnology

Background:

Telomerase is crucial for telomere maintenance and serves as a cancer biomarker.
Existing telomerase detection methods can be complex and lack direct visualization capabilities.

Purpose of the Study:

To introduce a novel telomerase detection strategy using DNA-PAINT.
To leverage DNA-PAINT's high spatial resolution for sensitive and direct visualization of telomere elongation.

Main Methods:

Development of a DNA-PAINT based strategy for telomerase detection.
Utilizing super-resolution imaging for high signal-to-noise ratio visualization.
Direct *in situ* observation of telomeric elongation processes.

Main Results:

Achieved extraordinary spatial resolution (approximately 10 nm).
Enabled sensitive detection and localization of telomeric elongation sites at the single-particle level.
Provided unprecedented direct visualization of the telomeric elongation process.

Conclusions:

The DNA-PAINT telomerase detection strategy offers significant advantages over traditional methods.
This technique facilitates dynamic investigation of telomerase-related physiological processes.
The method holds promise for improved cancer diagnosis and research.