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Updated: Oct 21, 2025
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A Net Mold-based Method of Scaffold-free Three-Dimensional Cardiac Tissue Creation
Published on: August 5, 2018
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Bioengineering of a scaffold-less three-dimensional tissue using net mould
Katsuhisa Sakaguchi1, Yusuke Tobe1, Jiayue Yang1
1Department of Integrative Bioscience and Biomedical Engineering, Graduate School of Advanced Science and Engineering, TWIns, Waseda University, 2-2 Wakamatsu-cho, Shinjuku-ku, Tokyo 162-8480, Japan.
Biofabrication
|September 6, 2021
Summary
This study introduces a novel scaffold-free tissue engineering method using a net metal mold. This technique successfully created strong, manipulable 3D tissues, advancing regenerative medicine and cultured meat production.
Area of Science:
- Biotechnology
- Regenerative Medicine
- Tissue Engineering
Background:
- Tissue engineering is crucial for regenerative medicine, drug screening, and cultured meat.
- Current biofabrication methods often rely on scaffolds and printing, facing limitations.
Purpose of the Study:
- To develop a novel scaffold-free tissue engineering method.
- To construct robust, easily manipulated 3D tissues.
- To explore the potential for next-generation tissue engineering products.
Main Methods:
- Normal human dermal fibroblasts were cultured to form spheroids (diameter ⩾200 μm).
- Spheroids were transferred to a net metal mold (gap ⩽100 μm) and shake-cultivated for weeks.
- Scaffold-free 3D tissue formation was achieved through spheroid fusion.
Main Results:
- Successfully constructed scaffold-free 3D tissues with significant manipulable strength.
- Demonstrated that tissue viability is highly dependent on the culture media used.
- Achieved unprecedented thickness in scaffold-free 3D tissue cultures.
Conclusions:
- The proposed method enables scaffold-free 3D tissue construction without conventional limitations.
- This technique offers a promising approach for developing advanced tissue engineering products.
- Further research into culture media optimization is warranted for enhanced tissue development.
