Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

1.1K
Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
1.1K
Oligosaccharide Assembly01:24

Oligosaccharide Assembly

3.1K
Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...
3.1K
MALDI-TOF Mass Spectrometry01:19

MALDI-TOF Mass Spectrometry

5.9K
Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.
Matrix-assisted laser desorption ionization (MALDI) is a commonly...
5.9K
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

7.3K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
7.3K
Mass Spectrum: Interpretation01:24

Mass Spectrum: Interpretation

1.8K
An unknown compound can be established by identifying the molecular ion peak in the mass spectrum. The molecular ion peak is often weak or absent due to the predominance of fragmentation in high-energy electron beams. In such cases, a low-energy electron beam can be used to scan the spectrum to enhance the intensity of the molecular ion peak. Additionally, chemical ionization, field ionization, and desorption ionization spectra are used to obtain a relatively intense molecular ion peak.
To...
1.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Topology Switching in Polymetallic Fragments Governed by Metal Encapsulation.

Journal of the American Chemical Society·2026
Same author

Plasmonic Nanocavity-Induced Degradation Pathway of Boronic Acid Biosensing Interfaces Revealed by <i>In Situ</i> Tip-Enhanced Raman Spectroscopy.

ACS nano·2026
Same author

Steroid Fingerprinting with Cryogenic Gas-Phase Infrared Spectroscopy.

ACS measurement science au·2026
Same author

Cryogenic Infrared Spectroscopy Unmasks Gas-Phase Charge Migration in Mucin-Type O-Glycans.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

Cryogenic Gas-phase IR Spectroscopy on a Commercial Ion Mobility-Mass Spectrometry Platform.

Analytical chemistry·2026
Same author

Bifunctional Lipid-Protein Cross-linking Efficiency and Reaction Products.

Journal of the American Chemical Society·2026
Same journal

Synthetic Porous Carbons for High-Energy, High-Power Supercapacitors.

Chemical reviews·2026
Same journal

Navigating Misfolded Terrain: ER-Associated Degradation of Membrane Proteins.

Chemical reviews·2026
Same journal

Ink Design for Printing Perovskite Solar Cells and Modules.

Chemical reviews·2026
Same journal

Advanced Single-Atom Catalysts for Thermal-Catalytic C1 Chemistry.

Chemical reviews·2026
Same journal

Copper-Dependent Polysaccharide Monooxygenases: Mechanism and Function.

Chemical reviews·2026
Same journal

To Biotic or Abiotic: Biohybrid Systems for Artificial Photosynthesis.

Chemical reviews·2026
See all related articles

Related Experiment Video

Updated: Oct 21, 2025

Mass Spectrometric Analysis of Glycosphingolipid Antigens
13:09

Mass Spectrometric Analysis of Glycosphingolipid Antigens

Published on: April 16, 2013

16.8K

Mass Spectrometry-Based Techniques to Elucidate the Sugar Code.

Márkó Grabarics1,2, Maike Lettow1,2, Carla Kirschbaum1,2

  • 1Institute of Chemistry and Biochemistry, Freie Universität Berlin, Arnimallee 22, 14195 Berlin, Germany.

Chemical Reviews
|September 7, 2021
PubMed
Summary
This summary is machine-generated.

Glycobiology, the study of sugars (glycans), is advancing rapidly. New mass spectrometry techniques are overcoming the complexity of sugar structures, unlocking the secrets of the "sugar code".

More Related Videos

Improved In-gel Reductive &#946;-Elimination for Comprehensive O-linked and Sulfo-glycomics by Mass Spectrometry
13:06

Improved In-gel Reductive β-Elimination for Comprehensive O-linked and Sulfo-glycomics by Mass Spectrometry

Published on: November 20, 2014

12.0K
Sequencing of Plant Wall Heteroxylans Using Enzymic, Chemical Methylation and Physical Mass Spectrometry, Nuclear Magnetic Resonance Techniques
11:49

Sequencing of Plant Wall Heteroxylans Using Enzymic, Chemical Methylation and Physical Mass Spectrometry, Nuclear Magnetic Resonance Techniques

Published on: March 24, 2016

7.8K

Related Experiment Videos

Last Updated: Oct 21, 2025

Mass Spectrometric Analysis of Glycosphingolipid Antigens
13:09

Mass Spectrometric Analysis of Glycosphingolipid Antigens

Published on: April 16, 2013

16.8K
Improved In-gel Reductive &#946;-Elimination for Comprehensive O-linked and Sulfo-glycomics by Mass Spectrometry
13:06

Improved In-gel Reductive β-Elimination for Comprehensive O-linked and Sulfo-glycomics by Mass Spectrometry

Published on: November 20, 2014

12.0K
Sequencing of Plant Wall Heteroxylans Using Enzymic, Chemical Methylation and Physical Mass Spectrometry, Nuclear Magnetic Resonance Techniques
11:49

Sequencing of Plant Wall Heteroxylans Using Enzymic, Chemical Methylation and Physical Mass Spectrometry, Nuclear Magnetic Resonance Techniques

Published on: March 24, 2016

7.8K

Area of Science:

  • Glycobiology and Mass Spectrometry
  • Carbohydrate Chemistry
  • Biomolecular Analysis

Background:

  • Cells use biopolymers like nucleic acids, proteins, and glycans to encode information.
  • Glycans are crucial for life, yet their biological roles and the 'sugar code' remain poorly understood.
  • The structural complexity of glycans presents significant analytical challenges, hindering glycobiology research.

Purpose of the Study:

  • To review recent advancements in mass spectrometry-based glycan analysis.
  • To highlight techniques that address the challenges of glycan structural complexity.
  • To provide insight into the structure and function of mammalian glycans.

Main Methods:

  • Innovative ion activation methods in mass spectrometry.
  • Ion mobility-mass spectrometry (IM-MS) commercialization and application.
  • Gas-phase ion spectroscopy and computational chemistry advancements.

Main Results:

  • These techniques have revolutionized mass spectrometry-based glycan analysis.
  • New methods provide unprecedented insight into complex carbohydrate structures.
  • Specific challenges in analyzing major mammalian glycan classes are discussed.

Conclusions:

  • Advancements in mass spectrometry are overcoming the inherent complexity of glycans.
  • The 'sugar code' is becoming more accessible for biological interpretation.
  • This review emphasizes the critical importance of glycans in biological systems.