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Biochemical Tools for Tracking Proteolysis.

Longxiang Wang1, Kimberly Main2,3,4, Henry Wang1

  • 1Department of Biochemistry, University of Alberta, Edmonton, AB T6G 2R3, Canada.

Journal of Proteome Research
|September 7, 2021
PubMed
Summary
This summary is machine-generated.

This study lists all known human proteases and presents tools to study their functions. Understanding these enzymes is crucial for developing new therapies for various diseases.

Keywords:
HPPLC-MS/MSMEROPSN-terminomicsneXtProtprotease inhibitorproteasesproteomics

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Last Updated: Oct 21, 2025

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Enzymology

Background:

  • Proteolysis, an irreversible post-translational modification, is vital for cellular processes.
  • Proteases, enzymes catalyzing protein hydrolysis, are classified into five mechanistic groups.
  • Dysregulated proteases significantly impact disease progression, yet many lack known substrates.

Purpose of the Study:

  • To provide a comprehensive catalog of human proteases.
  • To introduce biochemical tools for characterizing protease functions.
  • To facilitate the understanding of protease roles in health and disease.

Main Methods:

  • Compilation of known and putative human proteases.
  • Review of biochemical assays for in vitro, in vivo, and ex vivo analyses.
  • Discussion of tools for functional characterization.

Main Results:

  • A comprehensive list of 473 known and 90 putative human proteases is presented.
  • Various biochemical tools for protease characterization are detailed.
  • The study highlights the need for further research into protease functions.

Conclusions:

  • Accurate characterization of human proteases is essential for therapeutic development.
  • Novel tools enable a deeper understanding of protease activities in biological systems.
  • Defining protease functions will advance treatments for diseases linked to protease dysregulation.