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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Fluorescence detection methods for microfluidic droplet platforms
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Flow radiocytometry using droplet optofluidics.

Byunghang Ha1, Tae Jin Kim2, Ejung Moon2

  • 1Department of Radiation Oncology, Stanford University, Stanford, CA, 94305-5847, USA; Department of Mechanical Engineering, Stanford University, Stanford, CA, 94305-3011, USA.

Biosensors & Bioelectronics
|September 7, 2021
PubMed
Summary
This summary is machine-generated.

Flow radiocytometry (FRCM) enables single-cell analysis using radiotracers, overcoming limitations of fluorescent labels. This new method quantifies radiotracer uptake, revealing cellular metabolic heterogeneity in cancer cells.

Keywords:
Droplet microfluidicsFluorodeoxyglucoseOptofluidicsRadiochemistryRadiofluorogenesisSingle-cell analysis

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Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Cell Biology

Background:

  • Flow cytometry is crucial for analyzing cell populations but struggles with small molecule studies due to interfering fluorescent labels.
  • Radiotracers offer high specificity and minimal interference for tracking biochemical processes in cells.
  • Current methods lack the ability to analyze radiotracer uptake at the single-cell level with high throughput.

Purpose of the Study:

  • To introduce flow radiocytometry (FRCM), a novel method for single-cell analysis using radiotracers.
  • To enable the study of small molecule uptake and biochemical processes at the single-cell level.
  • To overcome the limitations of traditional flow cytometry in small molecule and biochemical studies.

Main Methods:

  • Development of flow radiocytometry (FRCM) utilizing droplet microfluidics and radiofluorogenesis.
  • Translation of single-cell radioactivity into a detectable fluorescent signal.
  • High-throughput optofluidic readout of the fluorescent signal for quantitative analysis.

Main Results:

  • Demonstrated proof of concept by quantifying [18F]fluorodeoxyglucose uptake in single human breast cancer cells.
  • Successfully assessed glucose metabolic flux and cellular heterogeneity at the single-cell level.
  • Validated FRCM's capability to analyze radiotracer uptake and metabolic activity.

Conclusions:

  • Flow radiocytometry (FRCM) expands the capabilities of cytometry for single-cell radiotracer studies.
  • FRCM provides a powerful tool for analyzing metabolic flux and heterogeneity in cell populations.
  • Potential applications include disease diagnostics, drug development, and analytical assays.