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Embolic Stroke Model with Magnetic Nanoparticles
Haoyuan Li1, Zhipeng Yang2, Qisheng Tang1
1Department of Neurosurgery, Huashan Hospital of Fudan University, 12 Wulumuqi Zhong Road, Shanghai 200040, China.
ACS Applied Materials & Interfaces
|September 8, 2021
Summary
Researchers developed a novel stroke model using magnetic nanoparticles (MNPs) and thrombin for embolic stroke research. This minimally invasive method effectively induces stroke and shows promising responses to thrombolytic drugs.
Area of Science:
- Neuroscience
- Biomedical Engineering
- Pharmacology
Background:
- Classic stroke models like suture occlusion have limitations, particularly for thrombolysis research.
- Existing embolic stroke models often involve complex surgical procedures.
- There is a need for simpler, more effective stroke models for pharmacological and rehabilitative studies.
Purpose of the Study:
- To develop a novel, minimally invasive stroke model in mice.
- To create an embolic stroke model suitable for thrombolysis research.
- To establish a reliable method for inducing cerebral artery occlusion.
Main Methods:
- Magnetic nanoparticles (MNPs) were cross-linked with thrombin to create MNP@Thrombin.
- A magnet was placed on the common carotid artery, and MNP@Thrombin was injected intravenously.
- Embolization and subsequent cerebral artery blockage were induced by MNP@Thrombin accumulation and clot formation.
Main Results:
- The MNP@Thrombin method successfully induced thrombus formation and blocked cerebral blood flow.
- The novel stroke model demonstrated significant responsiveness to thrombolytic drugs, such as urokinase.
- Cerebral blood flow was restored, and neurological symptom scores improved after thrombolytic treatment.
Conclusions:
- This new method provides a quick, easy, and minimally invasive stroke model in mice.
- The model effectively blocks hemisphere blood flow, damages neural function, and is suitable for pharmacological and rehabilitative research.
- The MNP@Thrombin approach offers a valuable tool for advancing stroke research, particularly in thrombolysis.
