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Updated: Oct 21, 2025
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Murine Excisional Wound Healing Model and Histological Morphometric Wound Analysis
Published on: August 21, 2020
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Longitudinal peripheral tissue RNA-Seq transcriptomic profiling, hyperalgesia, and wound healing in the rat plantar
Taichi Goto1, Matthew R Sapio2, Dragan Maric3
1Symptoms Biology Unit, National Institute of Nursing Research, National Institutes of Health, Bethesda, MD, USA.
Summary
Researchers identified key genes and cellular responses in a rat surgical incision model. This study reveals temporal molecular changes linked to pain and healing, offering potential therapeutic targets for wound management.
Area of Science:
- Wound healing research
- Molecular biology
- Pain management
Background:
- Surgical wound pain and delayed healing mechanisms are complex and understudied.
- Effective management strategies require a deeper understanding of the underlying biological processes.
Purpose of the Study:
- To investigate temporal changes in behavior, tissue structure, and gene expression following a surgical incision in a rat model.
- To identify specific molecular markers and pathways involved in early and late stages of wound healing and associated pain.
Main Methods:
- Utilized a rat model of surgical incision.
- Employed hyperalgesic behavioral tests, histological analyses, and next-generation RNA sequencing.
- Examined gene expression, leukocyte infiltration, and tissue structure at various time points post-incision.
Main Results:
- Chemokines Cxcl1 and Cxcl2 were rapidly expressed within 1 hour.
- Leukocyte infiltration peaked at 24 hours, coinciding with peak hyperalgesia and high transcriptional activity.
- Nppb (natriuretic peptide precursor B) was highly upregulated at 1 day and localized to epidermal keratinocytes.
- Specific peptidases (Mmp2, Aebp1, Mmp23, Adamts7, Adamtsl1) showed increased expression in the late healing phase.
Conclusions:
- Identified time-specific gene expression patterns in response to surgical incisions.
- Highlighted the roles of chemokines, leukocytes, Nppb, and specific peptidases in wound pain and healing.
- These identified transcripts represent potential biomarkers or therapeutic targets for improving wound pain and healing outcomes.
