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Related Experiment Videos

Fluorescent color factor calculation using dBASE-II.

R L King1, H A Carter, P J Birckbichler

  • 1Samuel Roberts Noble Foundation Inc., Biomedical Division, Ardmore, OK 73402.

Computer Applications in the Biosciences : CABIOS
|June 1, 1986
PubMed
Summary
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A new software system calculates amino acid and dipeptide limits using high-performance liquid chromatography data. This method provides acceptable ranges for quality control in biochemical analysis.

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Software Engineering

Background:

  • High-performance liquid chromatography (HPLC) is crucial for analyzing amino acids and peptides.
  • Accurate quality control requires defined acceptable limits for analytical parameters.
  • Existing software solutions may not be optimized for specific biochemical analyses.

Purpose of the Study:

  • To describe a novel software system for calculating analytical parameters in amino acid and dipeptide analysis.
  • To implement quality control measures by defining acceptable limits for retention times and color factors.
  • To provide a user-friendly software solution for researchers using HPLC.

Main Methods:

  • Development of a software system utilizing dBASE-II on an Apple II+ computer.

Related Experiment Videos

  • Application of high-performance liquid chromatography (HPLC) for the separation and detection of amino acids and a dipeptide.
  • Calculation of color factors and retention times for 15 amino acids and epsilon-(gamma-glutamyl)lysine.
  • Statistical analysis to determine acceptable limits (mean ± 2 standard deviations).

    • Main Results:

    • The software successfully calculates color factors and retention times for 15 amino acids and epsilon-(gamma-glutamyl)lysine.
    • Acceptable limits for these parameters are generated, providing a basis for quality control.
    • The software package is distributed in source code form, allowing for customization and further development.

    Conclusions:

    • The developed software system offers a practical tool for HPLC-based amino acid and dipeptide analysis.
    • The calculated acceptable limits enhance the reliability and reproducibility of biochemical analyses.
    • The availability of the source code promotes accessibility and potential integration into various laboratory workflows.