Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Peptide sequencing program.

C W Hamm1, W E Wilson, D J Harvan

  • 1Laboratory of Behavioral and Neurological Toxicology, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709.

Computer Applications in the Biosciences : CABIOS
|June 1, 1986
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Coronary intravascular lithotripsy and rotational atherectomy for severely calcified stenosis: Results from the ROTA.shock trial.

Catheterization and cardiovascular interventions : official journal of the Society for Cardiac Angiography & Interventions·2023
Same author

Instant rule-out of suspected non-ST-segment elevation myocardial infarction using high-sensitivity cardiac troponin T with Copeptin versus a single low high-sensitivity cardiac troponin T: findings from a large pooled individual data analysis on 10,329 patients.

Clinical research in cardiology : official journal of the German Cardiac Society·2020
Same author

[Prehospital management of acute coronary syndrome in patients on long-term direct oral anticoagulant treatment].

Der Anaesthesist·2019
Same author

Everolimus- vs. novolimus-eluting bioresorbable scaffolds in patients with acute coronary syndrome.

Herz·2019
Same author

[MINOCA-myocardial infarction with non-obstructive coronary arteries].

Herz·2018
Same author

[Patent foramen ovale-intervention or pharmaceutical treatment].

Der Internist·2018
Same journal

DCA: an efficient implementation of the divide-and-conquer approach to simultaneous multiple sequence alignment.

Computer applications in the biosciences : CABIOS·1998
Same journal

Two applications to facilitate the viewing of database search result files on the Macintosh.

Computer applications in the biosciences : CABIOS·1998
Same journal

BioWish: a molecular biology command extension to Tcl/Tk.

Computer applications in the biosciences : CABIOS·1998
Same journal

The Sequence Alerting Server--a new WEB server.

Computer applications in the biosciences : CABIOS·1998
Same journal

A software tool for the analysis of mass spectrometric disulfide mapping experiments.

Computer applications in the biosciences : CABIOS·1998
Same journal

SAMBA: hardware accelerator for biological sequence comparison.

Computer applications in the biosciences : CABIOS·1998
See all related articles

A new computer program automates peptide fragmentation analysis by generating all possible amino acid sequences from a known composition and matching them to experimental spectra.

Area of Science:

  • Biochemistry
  • Computational Biology
  • Mass Spectrometry

Background:

  • Peptide identification is crucial in proteomics.
  • Manual interpretation of peptide fragmentation patterns is complex and time-consuming.
  • Automated methods are needed to accelerate sequence analysis.

Purpose of the Study:

  • To develop a computational tool for automating the analysis of peptide fragmentation patterns.
  • To improve the efficiency and accuracy of peptide identification from mass spectrometry data.

Main Methods:

  • A computer program was developed to systematically generate all potential linear amino acid sequences based on a given peptide's amino acid composition.
  • The generated sequences were computationally searched against experimental mass spectrometry data.

Related Experiment Videos

  • Sequence matching algorithms were employed to identify the most probable peptide sequence.

    • Main Results:

    • The program successfully automates the rationalization of peptide fragmentation patterns.
    • It systematically generates and evaluates potential peptide sequences.
    • The method allows for the identification of unknown peptide sequences by matching generated possibilities to spectral data.

    Conclusions:

    • The developed computer program provides an automated approach for peptide fragmentation pattern analysis.
    • This tool can aid in the efficient and accurate identification of peptides in mass spectrometry-based proteomics.
    • Automation of this process enhances the throughput of proteomic research.