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Updated: Aug 17, 2026

Improved Swiss-rolling Technique for Intestinal Tissue Preparation for Immunohistochemical and Immunofluorescent Analyses
Published on: July 13, 2016
Changes of localization of lectin-binding sites on the intestinal epithelial cell surface membrane during
1Department of Anatomy, Histology and Embryology, Academy of Medicine, Sofia, Bulgaria.
Abstract:
Light and electron microscopically localizations of Concanavalin A, Soybean Agglutinin, and Asparagus Pea Lectin-binding sites on the intestinal microvillous membrane were studied by means of horseradish peroxidase labelled lectins. The distribution of the lectin-binding sites was composed with the cell differentiation from the crypts to the tip of the intestinal villi. The visualization of lectin receptors in the mucin vesicles in the goblet cells was established as well. The controls for the determination of the specificity of reactions were carried out.
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