Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

7.8K
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
7.8K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

17.6K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
17.6K
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

11.2K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
11.2K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

10.4K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
10.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hydroxyl-rich nanocavities on perovskite enable nearly barrierless intramolecular hydrogen transfer for nitrate electroreduction to ammonia.

Nature communications·2026
Same author

Template-Confined Synthesis of 1 nm High-Entropy-Alloy Nanoparticle Library for Electrocatalysis.

ACS nano·2026
Same author

Trifunctional electrocatalyst with accurate surface reconstruction for zinc-air batteries and water electrolyzers.

Nature communications·2026
Same author

Goblet-Like P-Driven Pt<sub>3</sub>Mn Alloys Enable High Power Density and 1000 h Durability in Practical Fuel Cells.

Journal of the American Chemical Society·2026
Same author

Volumetric Imaging of Ex Vivo Oral Mucosa Specimens with Multi-Scale Wide Field-of-View Optical Coherence Tomography/Microscopy in Near-Infrared-II Window.

Diagnostics (Basel, Switzerland)·2026
Same author

Structural Reconstruction and Electronic Microenvironment of Active FeCoNiOOH Support Optimized by Single Mo Atoms for Lattice Oxygen Evolution.

Small (Weinheim an der Bergstrasse, Germany)·2026

Related Experiment Video

Updated: Oct 20, 2025

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
12:54

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Published on: October 2, 2021

3.4K

Rapid pseudo-H&E imaging using a fluorescence-inbuilt optical coherence microscopic imaging system.

Sey-En Lin1,2, Dong-Yo Jheng1, Kuang-Yu Hsu1

  • 1AcuSolutions Inc., 3F., No. 2, Ln. 263, Chongyang Rd., Nangang Dist., Taipei, Taiwan.

Biomedical Optics Express
|September 13, 2021
PubMed
Summary
This summary is machine-generated.

A novel optical coherence microscopy technique creates fast, digital pseudo-H&E images from fresh tissue. This method accelerates cellular morphology assessment for biobanking and veterinary diagnostics without physical embedment.

More Related Videos

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT
12:22

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT

Published on: August 4, 2018

8.7K
High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging
13:49

High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging

Published on: January 11, 2011

34.7K

Related Experiment Videos

Last Updated: Oct 20, 2025

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
12:54

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Published on: October 2, 2021

3.4K
Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT
12:22

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT

Published on: August 4, 2018

8.7K
High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging
13:49

High-resolution Fiber-optic Microendoscopy for in situ Cellular Imaging

Published on: January 11, 2011

34.7K

Area of Science:

  • Biomedical Optics
  • Histopathology
  • Medical Imaging

Background:

  • Traditional Hematoxylin and Eosin (H&E) staining requires tissue fixation and processing, which can be time-consuming.
  • Assessing fresh tissue morphology is crucial for rapid diagnostics, particularly in veterinary medicine and biobanking.
  • Existing methods for fresh tissue imaging often lack the cellular detail and H&E-like appearance necessary for histopathological evaluation.

Purpose of the Study:

  • To develop and demonstrate a rapid, non-invasive imaging technique for cellular-level morphological assessment of fresh tissue specimens.
  • To generate digital pseudo-H&E (p-H&E) images comparable to traditional H&E staining.
  • To establish a faster alternative to conventional frozen-section H&E processing for specific tissue types.

Main Methods:

  • Utilized Linnik-based optical coherence microscopy (OCM) integrated with fluorescence microscopy (FM).
  • Employed color-coding and RGB composition to merge OCM and FM data, creating position-matched, digital p-H&E images.
  • Developed a workflow from fresh tissue receipt to stitched image generation in under 15 minutes for a 1 cm² specimen.

Main Results:

  • Successfully generated high-resolution, H&E-like images from fresh porcine and biobank tissue specimens.
  • Achieved processing times approximately two times faster than traditional frozen-section H&E for fatty or water-rich tissues.
  • Demonstrated the technique's applicability for both human and animal tissues, including detailed visualization of cellular structures and elastic fibers.

Conclusions:

  • The OCM-based technique provides a rapid and effective method for generating pseudo-H&E images from fresh tissue.
  • This approach offers a significant time advantage over conventional methods, particularly for challenging tissue types.
  • The technique is suitable for biobanking, veterinary diagnostics, and potentially intraoperative assessment, enhancing histopathological workflow efficiency.