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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Related Experiment Video

Updated: Jun 22, 2026

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM

Published on: June 28, 2017

Review: tomographic imaging flow cytometry.

Andreas Kleiber¹, Daniel Kraus¹, Thomas Henkel¹

¹Leibniz Institute of Photonic Technology, Albert-Einstein-Straße 9, D-07745 Jena, Germany.

|September 13, 2021

Summary

This summary is machine-generated.

Tomographic imaging flow cytometry (tIFC) advances traditional methods by providing 3D visualization of particles. This technique offers a holistic view of surfaces and internal structures, crucial for biological and healthcare applications.

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Last Updated: Jun 22, 2026

Microfluidic Imaging Flow Cytometry by Asymmetric-detection Time-stretch Optical Microscopy ATOM

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Area of Science:

Biomedical Engineering
Analytical Chemistry
Cell Biology

Background:

Conventional flow cytometry (FC) is a key diagnostic and research tool.
Imaging flow cytometry (IFC) enhances FC with high-resolution optical and spectroscopic data.
Standard IFC captures only 2D projections, limiting 3D structural analysis.

Purpose of the Study:

To review the development and applications of tomographic imaging flow cytometry (tIFC).
To highlight tIFC's capability in visualizing 3D particle information.
To provide an overview of current and historical advancements in tIFC technology.

Main Methods:

Review of existing literature on flow cytometry and imaging techniques.
Analysis of tomographic reconstruction principles applied to flow cytometry.
Examination of tIFC system designs and data processing methodologies.

Main Results:

tIFC overcomes the 2D limitation of IFC by enabling 3D reconstruction.
The technology allows for holistic visualization of particle surfaces and internal structures.
Significant advancements have been made in tIFC resolution and data acquisition.

Conclusions:

tIFC represents a significant evolution in flow cytometry, offering unprecedented 3D insights.
This technology holds great potential for enhancing biological research and clinical diagnostics.
Continued development in tIFC promises further improvements in cellular and particle analysis.