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Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
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Overexpression of hsa_circ_0022742 suppressed hyperglycemia-induced endothelial dysfunction by targeting the

Siyang Liu1, Liyun Wang1, Xueyan Wu2

  • 1Chengde Central Hospital, Chengde, Hebei 067000, China.

Microvascular Research
|September 13, 2021
PubMed

Insights

High glucose levels in diabetes damage blood vessels. This study shows that increasing hsa_circ_0022742 can protect against this damage by regulating the miR-503-5p/FBXW7 pathway.

Area of Science:

  • Endocrinology
  • Molecular Biology
  • Vascular Biology

Background:

  • Diabetes mellitus (Type I and II) negatively impacts organ microvasculature.
  • Hyperglycemia (HG)-induced endothelial dysfunction is linked to altered circular RNA (circRNA) expression.
  • The precise regulatory mechanisms underlying these effects remain incompletely understood.

Purpose of the Study:

  • To investigate the role of circRNAs in hyperglycemia-induced endothelial dysfunction.
  • To elucidate the molecular mechanism of hsa_circ_0022742 in protecting against HG-induced endothelial damage.
  • To identify downstream targets of hsa_circ_0022742.

Main Methods:

  • High-throughput sequencing of circRNAs in human umbilical vein endothelial cells (HUVECs) under HG conditions.
  • Bioinformatics analysis, luciferase reporter assays, and qRT-PCR to validate molecular interactions.
  • Flow cytometry and angiogenic differentiation assays to assess endothelial cell function.

Main Results:

  • HG treatment decreased hsa_circ_0022742 expression in HUVECs.
  • Overexpression of hsa_circ_0022742 ameliorated HG-induced endothelial dysfunction, including reduced apoptosis and inflammation.
  • hsa_circ_0022742 targets miR-503-5p and FBXW7, enhancing FBXW7 expression by sponging miR-503-5p.

Conclusions:

  • hsa_circ_0022742 plays a protective role against hyperglycemia-induced endothelial dysfunction.
  • The miR-503-5p/FBXW7 axis is a key downstream pathway regulated by hsa_circ_0022742.
  • Targeting the hsa_circ_0022742/miR-503-5p/FBXW7 pathway may offer therapeutic potential for diabetic microvascular complications.

Keywords:
Endothelial cellFBXW7Hyperglycemiahsa_circ_0022742miR-503-5p

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