Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Notch Signaling Pathway03:14

Notch Signaling Pathway

4.6K
The Notch signaling pathway is a major intracellular signaling pathway that is highly conserved over a broad spectrum of metazoan species. It stands unique from other intracellular signaling mechanisms in animals because notch protein itself acts as the receptor as well as the primary signaling molecule.
The Notch gene came into the limelight in 1914 after the discovery that its mutation in Drosophila melanogaster leads to a serrated (or "notched") wing margin phenotype. It was not...
4.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

FGFR signaling establishes spatial gradients of secretory cell identities along the airway proximal-distal axis.

Nature communications·2026
Same author

Insights and emerging perspectives from the 2025 Medaka PI/ERC IndiGene meeting: Heidelberg July 22-24, 2025.

Developmental dynamics : an official publication of the American Association of Anatomists·2026
Same author

The Role of Self-Care Strategies and Workplace Support on Burnout Among Case Managers.

Professional case management·2026
Same author

Spatial single-cell atlas reveals regional variations in healthy and diseased human lung.

Nature communications·2025
Same author

Cloning-Free Targeting of Endogenous Loci to Generate Fluorescent Reporters in Medaka.

Bio-protocol·2025
Same author

Creating Opioid Response Specialists: A Harm Reduction Initiative.

Prehospital emergency care·2025

Related Experiment Video

Updated: Oct 20, 2025

Drug Treatment and In Vivo Imaging of Osteoblast-Osteoclast Interactions in a Medaka Fish Osteoporosis Model
08:53

Drug Treatment and In Vivo Imaging of Osteoblast-Osteoclast Interactions in a Medaka Fish Osteoporosis Model

Published on: January 1, 2017

9.4K

Local tissue interactions govern pLL patterning in medaka.

Ali Seleit1, Karen Gross1, Jasmin Onistschenko1

  • 1Laboratory of Clonal Analysis of Post-Embryonic Stem Cells, Centre for Organismal Studies (COS) Heidelberg, Im Neuenheimer Feld 230, Universität Heidelberg, 69120, Heidelberg, Germany; Heidelberg Biosciences International Graduate School (HBIGS), Universität Heidelberg, Heidelberg, Germany.

Developmental Biology
|September 13, 2021
PubMed
Summary

Epithelial integrity, not neuronal wiring, guides sensory organ positioning in medaka fish. This finding clarifies how diverse body plans accommodate sensory systems, revealing crucial tissue interactions in development.

Keywords:
Epithelial cellsKeratinKeratin 15Lateral lineMedakaPattern formationSensory system

More Related Videos

Sperm Collection and Computer-Assisted Sperm Analysis in the Teleost Model Japanese Medaka Oryzias latipes
06:49

Sperm Collection and Computer-Assisted Sperm Analysis in the Teleost Model Japanese Medaka Oryzias latipes

Published on: October 6, 2022

4.3K
A Microfluidics Approach for the Functional Investigation of Signaling Oscillations Governing Somitogenesis
08:06

A Microfluidics Approach for the Functional Investigation of Signaling Oscillations Governing Somitogenesis

Published on: March 19, 2021

3.0K

Related Experiment Videos

Last Updated: Oct 20, 2025

Drug Treatment and In Vivo Imaging of Osteoblast-Osteoclast Interactions in a Medaka Fish Osteoporosis Model
08:53

Drug Treatment and In Vivo Imaging of Osteoblast-Osteoclast Interactions in a Medaka Fish Osteoporosis Model

Published on: January 1, 2017

9.4K
Sperm Collection and Computer-Assisted Sperm Analysis in the Teleost Model Japanese Medaka Oryzias latipes
06:49

Sperm Collection and Computer-Assisted Sperm Analysis in the Teleost Model Japanese Medaka Oryzias latipes

Published on: October 6, 2022

4.3K
A Microfluidics Approach for the Functional Investigation of Signaling Oscillations Governing Somitogenesis
08:06

A Microfluidics Approach for the Functional Investigation of Signaling Oscillations Governing Somitogenesis

Published on: March 19, 2021

3.0K

Area of Science:

  • Developmental Biology
  • Neuroscience
  • Genetics

Background:

  • Vertebrate organ positioning is species-specific, with significant variation in teleosts.
  • Understanding how diverse body plans accommodate organ systems remains a challenge.
  • The posterior lateral line (pLL) system in medaka fish offers a model for studying pattern formation.

Purpose of the Study:

  • To investigate the tissue interactions that establish organ patterning in the medaka pLL system.
  • To determine the role of epithelial integrity versus neuronal wiring in sensory organ placement.
  • To elucidate the mechanisms underlying morphological adaptation in teleost sensory systems.

Main Methods:

  • Analysis of neuromast arrangement in the medaka posterior lateral line (pLL) system.
  • Generation and analysis of keratin 15 (siml) mutants exhibiting epithelial lesions.
  • Utilizing siml/wild-type chimeras and 2-photon laser ablation during pLL morphogenesis.

Main Results:

  • pLL patterning is established by autonomous organ precursors, independent of neuronal connections.
  • Mutant epithelium in siml mutants disrupts pLL patterning, but wild-type epithelium rescues the phenotype.
  • Epithelial lesions induced by laser ablation phenocopy genetic mutants, confirming epithelial integrity's role in neuromast positioning.

Conclusions:

  • Epithelial integrity, rather than neuronal wiring, is critical for establishing the precise positioning of embryonic pLL neuromasts.
  • Intrinsic programs ensure organ morphogenesis, while extrinsic tissue interactions facilitate adaptation to diverse body plans.
  • This study disentangles intrinsic and extrinsic factors in sensory system development within the context of teleost diversity.