Related Experiment Video

Updated: Oct 20, 2025

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal
08:01

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal

Published on: May 30, 2012

10.6K

Emerging roles of bromodomain protein 4 in regulation of stem cell identity

Anusree Dey1,2, Sheetal Uppal1,2, Jayeeta Giri3

  • 1Molecular Biology Division, Bhabha Atomic Research Centre, Mumbai, India.

Stem Cells (Dayton, Ohio)
|September 14, 2021
PubMed

Insights

Bromodomain protein 4 (BRD4) is crucial for stem cell potency and lineage commitment. Understanding BRD4

Area of Science:

  • Stem cell biology and epigenetics.
  • Molecular mechanisms of cell fate determination.

Background:

  • Stem cell fate decisions and lineage commitment are critical for regenerative medicine.
  • Epigenetic reprogramming and transcriptional changes govern stem cell potency.
  • Bromodomain protein 4 (BRD4) acts as an epigenetic reader, regulating gene expression and RNA polymerase II elongation.

Purpose of the Study:

  • To review the role of BRD4 in stem cell fate decisions and lineage commitment.
  • To elucidate BRD4's interplay with pluripotency factors in controlling stem cell transitions.
  • To highlight BRD4 as a druggable target for stem cell therapeutics.

Main Methods:

  • Systematic review of scientific literature.
  • Analysis of BRD4's function in gene regulatory networks.
  • Focus on stem cell differentiation, reprogramming, and transdifferentiation.

Main Results:

  • BRD4 plays a significant role in maintaining stem cell potency.
  • Dysregulation of BRD4 can lead to uncontrolled cell proliferation and tumor formation.
  • BRD4 mediates epigenetic regulation crucial for stem cell fate.

Conclusions:

  • BRD4 is a key regulator of stem cell potency and lineage commitment.
  • Targeting BRD4 offers potential for novel stem cell-based therapies.
  • Further understanding of BRD4's mechanisms can advance regenerative medicine.
Keywords:
BRD4epigeneticspluripotencyregenerative-medicinestem cellstranscription

More Related Videos

Sonication-facilitated Immunofluorescence Staining of Late-stage Embryonic and Larval Drosophila Tissues In Situ10:10

Sonication-facilitated Immunofluorescence Staining of Late-stage Embryonic and Larval Drosophila Tissues In Situ

Published on: August 14, 2014

12.8K
A Simple Method to Identify Kinases That Regulate Embryonic Stem Cell Pluripotency by High-throughput Inhibitor Screening07:18

A Simple Method to Identify Kinases That Regulate Embryonic Stem Cell Pluripotency by High-throughput Inhibitor Screening

Published on: May 12, 2017

6.6K

Related Concept Videos

Maintenance of the ES Cell State01:14

Maintenance of the ES Cell State

The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
2.3K
Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.3K
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.2K
Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
1.9K
Stem Cell Niche01:26

Stem Cell Niche

The stem cell niche is the dynamic microenvironment where stem cells reside. Inside these niches, the cells may remain undifferentiated, undergo high self-renewal, or become lineage-specific progenitors. Stem cells coexist with other niche cells, such as stromal cells. They also interact closely with the ECM. Cell-cell and cell-matrix communication occur via adhesion molecules or soluble factors that signal the stem cells and determine their fate. Stromal cells also provide survival signals to...
5.5K
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.2K