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Rat intestinal brush border enzymes release by deoxycholate in vivo
Pflugers Archiv : European Journal of Physiology
|February 22, 1978
Summary
Sodium deoxycholate (DOC) releases proteins and hydrolases from the rat jejunum. Different enzymes, like sucrase (SA) and alkaline phosphatase (AP), showed distinct release patterns and solubilization, suggesting varied brush border membrane localization.
Area of Science:
- Gastroenterology
- Biochemistry
- Cell Biology
Background:
- The jejunum's brush border membrane contains various hydrolases crucial for digestion.
- Understanding the release mechanisms of these enzymes is important for comprehending intestinal function and disease.
Purpose of the Study:
- To investigate the effect of sodium deoxycholate (DOC) on the release and solubilization of proteins and specific hydrolases from the rat jejunum in vivo.
- To determine the differential release patterns and localization of sucrase (SA) and alkaline phosphatase (AP) within the brush border membrane.
Main Methods:
- An in vivo perfusion technique was employed using rat jejunum.
- Sodium deoxycholate (DOC) at 2 mmol/L was perfused for 30 minutes.
- Analysis of protein and hydrolase release, specific enzyme activities, and solubilization states was performed.
Main Results:
- DOC perfusion induced a marked, reversible release of proteins and hydrolases.
- Sucrase (SA) release significantly increased, while alkaline phosphatase (AP) release decreased.
- Enzymes exhibited distinct release patterns and solubilization; SA was mainly soluble, AP in a particular form.
- Microscopy showed minimal epithelial cell exfoliation without specific pathological changes.
Conclusions:
- Enzyme release and solubilization by DOC vary, indicating differential localization within the brush border membrane.
- Sucrase (SA) appears to be located superficially, whereas alkaline phosphatase (AP) is more deeply embedded and less accessible.