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A gain series method for accurate EMCCD calibration.

Duncan P Ryan¹, Megan K Dunlap², Martin P Gelfand³

¹Center for Integrated Nanotechnologies, Los Alamos National Laboratory, Los Alamos, 87545, USA. ryand@lanl.gov.

Scientific Reports

|September 16, 2021

Summary

Accurate photon counting requires calibrating electron-multiplying charge-coupled device (EMCCD) cameras. A new gain-series calibration method quickly and accurately estimates EMCCD gain response, outperforming other approaches in self-consistency tests.

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Area of Science:

Scientific Imaging
Optical Engineering
Photonics

Background:

Accurate photon counting with electron-multiplying charge-coupled device (EMCCD) cameras necessitates precise calibration of gain and digital conversion factor.
Existing calibration methods may lack speed or accuracy across multiple gain settings.

Purpose of the Study:

To introduce a novel, rapid method for calibrating multiple gain settings of an EMCCD camera.
To enhance the accuracy of estimating the camera's gain response.

Main Methods:

Acquisition of gain-series calibration data.
Analysis of acquired images using the established EMCCD noise model.
Development of a comparative framework to evaluate different calibration techniques.

Main Results:

The proposed gain-series calibration method provides a more accurate estimation of the EMCCD camera's gain response.
Self-consistency tests demonstrate that gain-series calibration surpasses other tested calibration approaches.

Conclusions:

The novel gain-series calibration method offers a significant improvement in accuracy and efficiency for EMCCD camera calibration.
This technique is crucial for applications demanding precise photon counting and quantitative imaging.